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ABSTRACT This study explores how suppressing asexual development inAspergillus nidulansenhances the mechanical properties of mycelial materials. Using four aconidial mutants(ΔbrlA, ΔflbA, ΔfluG, andfadA^G42R) that lack asexual development and a control strain (A28) that undergoes typical asexual development, we found that the absence of asexual development significantly improves mechanical strength. All mutants exhibited higher ultimate tensile strength (UTS) than the control, with ΔfluGand ΔbrlA(fluffy nonsporulating, FNS phenotype) showing the highest UTS. Additionally,fadA^G42Rand ΔflbA(fluffy autolytic dominant, FAD phenotype) demonstrated significantly higher strain at failure (SF), linked to increased autolysis and lower dry cell mass compared to the control and FNS mutants. Solid-state NMR analysis revealed that autolysis in FAD mutants disrupts galactofuranose-related metabolic processes, altering cell wall composition and contributing to higher elasticity. These findings suggest that suppressing asexual development enhances mycelial material strength, while autolysis mechanisms influence elasticity. This research highlights the potential for genetic manipulation in fungi to engineer advanced mycelial-based materials with tailored mechanical properties.